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KMID : 0363819920260020346
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Korean Journal of Nuclear Medicine
1992 Volume.26 No. 2 p.346 ~ p.354
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Establishment of I-131, Tc-99m Labeling Methods to In-house Anti-CEA Antibodies and Evaluation of the Immunological
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정준기
이동수/이명철/정홍근/고창순/홍미경/최석례/서일택/정준호
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Abstract
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Cancer cells have several tumor-associated antigens on the cell surfaces, and antibodies against these antigens have been developed by many investigators. Radiolabeled antibodies have been used as new methods to diagnose and treat malignant
tumors.
Especially anti-carcinoembryonic antigen (CEA) is the most popular antibody for these purposes.
In this investigation, we tried to label 131I and 99mTc to anti-CEA monoclonal antibodies which were developed in the Seoul National University College of Medicine. We found CEA-79 and CEA-92 antibodies had the better immunological
characteristics
among
8 anti-CEA monoclonal antibodies. And radioiodination of CEA-92 by iodogen method.
To label these antibodies with 99mTc, we used pretargeting transchelation as direct labeling method. At first, 99mTc was bound to glucaric acid, and monoclonal antibody was reduced by β-mercaptoethanol. When these were incubated together, 99mTc
bound
to glucarate was switched to monoclonal antibody because of higher affinity. We established conditions of several steps in this method.
Anti-CEA monoclonal antibodies labeled with 131I and 99mTc are expected to be used valuably in the detection and treatment of malignant tumors.
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KEYWORD
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